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ge sample loading reagent  (fluidigm)


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    Structured Review

    fluidigm ge sample loading reagent
    Ge Sample Loading Reagent, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 190 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ge+sample+loading+reagent/GE+Sample+Loading+Reagent/pm41744684-126-25-29
    Average 94 stars, based on 190 article reviews
    ge sample loading reagent - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Development of a multi-targeted real-time PCR assay for the detection of the grapevine pathogen Xylophilus ampelinus
    Article Snippet: The assay mixtures were prepared with the following components per sample: 3 μL 2× Assay Loading Reagent (Standard BioTools, PN 85000736) and 3 μL 20× mixture of primers and probe. .. The sample premix was prepared with the following components: 3 μL of TaqMan Universal PCR Master Mix (Applied Biosystems), 0.3 μL of 20× GE Sample Loading Reagent (Standard BioTools) and 2.7 μL of bacterial DNA. .. The 48.48DA was primed with the IFC Controller MX (Standard BioTools) prior to loading the samples and assays.

    Article Title: Modulation of lipid composition and gene expression by CNP supplementation in in vitro cultured bovine embryos.
    Article Snippet: .. For gene expression analysis, the sample solution preparation consisted of 2.25 mL cDNA (preamplified products), 2.5 mL of TaqMan Universal PCR Master Mix (2×, Applied Biosystems), and 0.25 mL of 20× GE Sample Loading Reagent (Fluidigm, South San Francisco, CA, United States); the assay solution included 2.5 mL 20× TaqMan Gene Expression Assay (Applied Biosystems) and 2.5 mL of 2× Assay Loading Reagent (Fluidigm). .. The 96.96 Dynamic ArrayTM Integrated Fluidic Circuits (Fluidigm) chip was used for data collection.

    Article Title: Modulation of lipid composition and gene expression by CNP supplementation in in vitro cultured bovine embryos
    Article Snippet: .. For gene expression analysis, the sample solution preparation consisted of 2.25 mL cDNA (preamplified products), 2.5 mL of TaqMan Universal PCR Master Mix (2×, Applied Biosystems), and 0.25 mL of 20× GE Sample Loading Reagent (Fluidigm, South San Francisco, CA, United States); the assay solution included 2.5 mL 20× TaqMan Gene Expression Assay (Applied Biosystems) and 2.5 mL of 2× Assay Loading Reagent (Fluidigm). .. The 96.96 Dynamic ArrayTM Integrated Fluidic Circuits (Fluidigm) chip was used for data collection.

    Article Title: In vivo targeted and deterministic single-cell malignant transformation
    Article Snippet: Quantitative PCR was performed using the high-throughput platform BioMark HD System and the 48.48 GE Dynamic Arrays (Standard Biotools). .. 6 μL of sample master mix (SMM) consisted of 1.8 μL of 5× diluted pre-amplified cDNA, 0.3 μL of 20× GE Sample Loading Reagent (Standard Biotools), and 3 μL of TaqMan Gene Expression PCR Master Mix (Life Technologies, Thermo Fisher). .. Each 6 μL assay master mix (AMM) consisted of 3 μL of TaqMan Gene Expression assay 20× (Life Technologies) and 3 μL of 2× Assay Loading Reagent (Standard Biotools).

    Article Title: Influence of the follicular wave on gene expression and in vitro embryo production in cattle.
    Article Snippet: This study evaluated oocyte competence, gene expression, and in vitro embryo production (IVEP) in cattle, based on follicular waves.. Twenty Bos taurus taurus donors were subjected to ovulation synchronization, starting with intramuscular administration of 2 mg estradiol benzoate and a 1.9 g intravaginal progesterone device on a random estrous cycle day (ten days before synchronized ovulation; D-10).. After 3 days, the device was removed, and 150 μg of D-cloprostenol sodium, 300 IU of equine chorionic gonadotrophin, and 1.0 mg of estradiol cypionate were administered.

    Article Title: In vivo targeted and deterministic single cell malignant transformation
    Article Snippet: Quantitative PCR was performed using the high- throughput platform BioMark HD System and the 48.48 GE Dynamic Arrays (Standard Biotools). .. 6 μL of sample master mix (SMM) consisted of 1.8 μL of 5× diluted pre- amplified cDNA, 0.3 μL of 20× GE Sample Loading Reagent (Standard Biotools), and 3 μL of TaqMan Gene Expression PCR Master Mix (Life Technologies, Thermo Fisher). .. Each 6 μL assay master mix (AMM) consisted of 3 μL of TaqMan Gene Expression assay 20× (Life Technologies) and 3 μL of 2× Assay Loading Reagent (Standard Biotools).

    Article Title: Novel translational mouse models of metabolic dysfunction-associated steatotic liver disease comparable to human MASLD with severe obesity
    Article Snippet: For amplification, we used the PreAmp master Mix (Fluidigm, PN 100–5580) according to the manufacture guidelines (Real-Time PCR user guide for the Fast gene Expression Analysis using TaqMan gene Expressions Assays). .. For PCR, we used the 48.48 Dynamic Array TM IFC, Assay loading reagent (Fluidigm, PN 85000736), GE Sample Loading Reagent (Fluidigm, PN 85000735, 85000746) and the Master Mix (TaqMan Gene Expression Master Mic, Applied Bioscience, PN 4369016) according to the manufactures guidelines. ..

    Gene Expression:

    Article Title: Modulation of lipid composition and gene expression by CNP supplementation in in vitro cultured bovine embryos.
    Article Snippet: .. For gene expression analysis, the sample solution preparation consisted of 2.25 mL cDNA (preamplified products), 2.5 mL of TaqMan Universal PCR Master Mix (2×, Applied Biosystems), and 0.25 mL of 20× GE Sample Loading Reagent (Fluidigm, South San Francisco, CA, United States); the assay solution included 2.5 mL 20× TaqMan Gene Expression Assay (Applied Biosystems) and 2.5 mL of 2× Assay Loading Reagent (Fluidigm). .. The 96.96 Dynamic ArrayTM Integrated Fluidic Circuits (Fluidigm) chip was used for data collection.

    Article Title: Modulation of lipid composition and gene expression by CNP supplementation in in vitro cultured bovine embryos
    Article Snippet: .. For gene expression analysis, the sample solution preparation consisted of 2.25 mL cDNA (preamplified products), 2.5 mL of TaqMan Universal PCR Master Mix (2×, Applied Biosystems), and 0.25 mL of 20× GE Sample Loading Reagent (Fluidigm, South San Francisco, CA, United States); the assay solution included 2.5 mL 20× TaqMan Gene Expression Assay (Applied Biosystems) and 2.5 mL of 2× Assay Loading Reagent (Fluidigm). .. The 96.96 Dynamic ArrayTM Integrated Fluidic Circuits (Fluidigm) chip was used for data collection.

    Article Title: Synergy between HA cleavage site sequence and NA-mediated plasminogen recruitment as a virulence mechanism for low-pathogenic avian influenza.
    Article Snippet: Assay mixes were prepared with 2.25 μL 2× assay loading reagent (Fluidigm), 2.5 μL of primer pair mix (1.15 μM), and 0.25 μL low EDTA TE buffer. .. Sample mixes were prepared with 2.5 μL TaqMan Gene Expression Master Mix (Thermo Scientific), 0.25 μL 20× EvaGreen DNA binding dye (Biotum), 0.25 μL 20× GE Sample Loading reagent (Fluidigm), and 2 μL of pre-amplified, Exonuclease-treated cDNA. ..

    Article Title: In vivo targeted and deterministic single-cell malignant transformation
    Article Snippet: Quantitative PCR was performed using the high-throughput platform BioMark HD System and the 48.48 GE Dynamic Arrays (Standard Biotools). .. 6 μL of sample master mix (SMM) consisted of 1.8 μL of 5× diluted pre-amplified cDNA, 0.3 μL of 20× GE Sample Loading Reagent (Standard Biotools), and 3 μL of TaqMan Gene Expression PCR Master Mix (Life Technologies, Thermo Fisher). .. Each 6 μL assay master mix (AMM) consisted of 3 μL of TaqMan Gene Expression assay 20× (Life Technologies) and 3 μL of 2× Assay Loading Reagent (Standard Biotools).

    Article Title: In vivo targeted and deterministic single cell malignant transformation
    Article Snippet: Quantitative PCR was performed using the high- throughput platform BioMark HD System and the 48.48 GE Dynamic Arrays (Standard Biotools). .. 6 μL of sample master mix (SMM) consisted of 1.8 μL of 5× diluted pre- amplified cDNA, 0.3 μL of 20× GE Sample Loading Reagent (Standard Biotools), and 3 μL of TaqMan Gene Expression PCR Master Mix (Life Technologies, Thermo Fisher). .. Each 6 μL assay master mix (AMM) consisted of 3 μL of TaqMan Gene Expression assay 20× (Life Technologies) and 3 μL of 2× Assay Loading Reagent (Standard Biotools).

    Article Title: Novel translational mouse models of metabolic dysfunction-associated steatotic liver disease comparable to human MASLD with severe obesity
    Article Snippet: For amplification, we used the PreAmp master Mix (Fluidigm, PN 100–5580) according to the manufacture guidelines (Real-Time PCR user guide for the Fast gene Expression Analysis using TaqMan gene Expressions Assays). .. For PCR, we used the 48.48 Dynamic Array TM IFC, Assay loading reagent (Fluidigm, PN 85000736), GE Sample Loading Reagent (Fluidigm, PN 85000735, 85000746) and the Master Mix (TaqMan Gene Expression Master Mic, Applied Bioscience, PN 4369016) according to the manufactures guidelines. ..

    Binding Assay:

    Article Title: Synergy between HA cleavage site sequence and NA-mediated plasminogen recruitment as a virulence mechanism for low-pathogenic avian influenza.
    Article Snippet: Assay mixes were prepared with 2.25 μL 2× assay loading reagent (Fluidigm), 2.5 μL of primer pair mix (1.15 μM), and 0.25 μL low EDTA TE buffer. .. Sample mixes were prepared with 2.5 μL TaqMan Gene Expression Master Mix (Thermo Scientific), 0.25 μL 20× EvaGreen DNA binding dye (Biotum), 0.25 μL 20× GE Sample Loading reagent (Fluidigm), and 2 μL of pre-amplified, Exonuclease-treated cDNA. ..

    Amplification:

    Article Title: In vivo targeted and deterministic single cell malignant transformation
    Article Snippet: Quantitative PCR was performed using the high- throughput platform BioMark HD System and the 48.48 GE Dynamic Arrays (Standard Biotools). .. 6 μL of sample master mix (SMM) consisted of 1.8 μL of 5× diluted pre- amplified cDNA, 0.3 μL of 20× GE Sample Loading Reagent (Standard Biotools), and 3 μL of TaqMan Gene Expression PCR Master Mix (Life Technologies, Thermo Fisher). .. Each 6 μL assay master mix (AMM) consisted of 3 μL of TaqMan Gene Expression assay 20× (Life Technologies) and 3 μL of 2× Assay Loading Reagent (Standard Biotools).



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